Ovation® RNA-Seq System V2
Our latest generation Ovation RNA-Seq System V2 provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications, now with enhanced transcript coverage and uniform distribution of sequencing reads. Amplification is initiated at the 3' end as well as randomly throughout the whole transcriptome in the sample. This feature makes the Ovation RNA-Seq System V2 ideal for amplification prior to…
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Microarrays have always worked out great because of this product.
Microarray
Self explanatory protocol, with suggestions, tips and tricks at each step to give best results. It allows for input for various sources and yields superior amplified product that can be used in applications that are sensitive to the quality of DNA. The results are reproducible and help in easy analysis, which is essential when projects run for years and data is generated from different batches of both samples and analytes.
Review Date: 14 Nov 2019 | NuGEN
Efficient, easy to use DNA library prep.
Low input DNA library prep
NuGEN's ultralow DNA library prep has been a life savor for low input DNA libraries, particularly ChIP. Even on samples that are below detection for standard QC methods, we can generate ample, high quality library the produces reliable data. V2 goes into the picogram range meaning there is virtually no sample input that it cannot handle. Plus you can go from DNA to ready to sequence, QC-ed library in less than a day.
Review Date: 25 Oct 2018 | NuGEN
Our latest generation Ovation RNA-Seq System V2 provides a fast and simple method for preparing amplified cDNA from total RNA for RNA-Seq applications, now with enhanced transcript coverage and uniform distribution of sequencing reads.
Amplification is initiated at the 3' end as well as randomly throughout the whole transcriptome in the sample. This feature makes the Ovation RNA-Seq System V2 ideal for amplification prior to Next Generation Sequencing, as it allows amplification of both mRNA and non-polyadenylated transcripts.
After purification, the cDNA can be fragmented to the appropriate size and ligated into a sequencing library using an Encore® Rapid Library System or Ovation® Ultralow Library System or other suitable library construction method.
The Ovation RNA-Seq System V2 is enabled by Ribo-SPIA technology, a rapid, simple and sensitive RNA amplification process developed by NuGEN. Using Ribo-SPIA technology and starting with as little as 500 pg total RNA, microgram quantities of cDNA can be prepared in approximately 4.5 hours.
The Ovation RNA-Seq System V2 (Part No. 7102) provides optimized reagent mixes and a protocol to process 8 or 32 total RNA samples, and is also available as an automation solution for processing 96 samples.
