pA-Tn5 Transposase - loaded
pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay.
pA-Tn5 Transposase - loaded
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Super-useful, time & cost-effective reagent for tagmentation-based assays!
epigenomics
The preloaded pAG-Tn5 from Diagenode is a very valuable consumables for those who attempt to perform tagmentation-based assays in the Epigenomics space. The enzyme is delivered as ready-to-use, pre-loaded with adaptors enzyme in glycerol-based storage buffer. This saves a considerable amount of time and provides with better reproducibility in terms of tagmentation efficiency. The enzyme is really active and performs well at the indicated dilution. However, we noticed that even further dilutions down to 1:500 v/v could be successfully used, which might be desirable given the high cost/quantity of this item. The support the user receives from the provider is great and they are very open to discuss also new applications of their products, providing with useful documentation and guidelines.
Review Date: 29 Sept 2023 | Diagenode
pA-Tn5 transposase is a fusion protein of hyperactive Tn5 transposase and protein A developed for the CUT&Tag assay. For convenience, the fusion protein is pre-loaded with sequencing adapters. Diagenode pA-Tn5 transposase is compatible with CUT&Tag and other antibody-tethered Tn5 based methods, like ACT-seq and CoBATCH.
pA-Tn5 Transposase
- High enrichment and specificity of the signal around the TSS
- No contamination with E. coli DNA
- Flexible versions: loaded or unloaded
- Excellent for CUT&Tag
As it is crucial to target only the protein of interest, a highly specific antibody is critical for the success of the CUT&Tag assay. All Diagenode ChIP-seq grade antibodies are validated for specificity using strict validation criteria to assure high performance in CUT&Tag.
Check out all products recommended for CUT&Tag assay:
- iDeal CUT&Tag kit for Histones - compatible with histome marks and some transcription factors
- pA/Tn5 Transposase (loaded or unloaded)
- CUT&Tag grade and ChIP-seq grade antibodies
- Negative & positive CUT&Tag controls: Antibody Package for CUT&Tag: anti-rabbit and anti-mouse
- DNA purification: IPure kit v2 or MicroChIP DiaPure columns
- Sequencing indexes: single indexes and UDI
