PCSK9 Mouse
PCSK9 mice were created utilizing a full-length human PSK9 cDNA with a C-terminal V5 tag expressed under the transcriptional control of a composite human albumin promoter containing a 235 bp SV40 enhancer and a 213 bp albumin promoter element. A chimeric intron was inserted between the promoter and PCSK 9 cDNA to enhance expression. The expression cassette was flanked by a 1.2 kb insulator sequence from the chicken beta-globu…
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PCSK9 mice were created utilizing a full-length human PSK9 cDNA with a C-terminal V5 tag expressed under the transcriptional control of a composite human albumin promoter containing a 235 bp SV40 enhancer and a 213 bp albumin promoter element.
A chimeric intron was inserted between the promoter and PCSK 9 cDNA to enhance expression. The expression cassette was flanked by a 1.2 kb insulator sequence from the chicken beta-globulin gene at both the 5’ and 3’ ends of the transgene. The expression construct was injected into the pronucleus of fertilized eggs from C57BL6J/N mice and the resulting founders and their transgenic offspring were identified from tail tissue by a PCR product amplified from the insulator sequence. Transferred to Charles River under exclusive license from Pfizer Inc., in 2011.
Model Properties:
- Coat Color Black
- Ideal For: Cholesterol regulation
- Strain Code: 531 HE, 532 HO, 533 WT, This model is cryopreserved.
