PDS-1000/He™ System
100/120 V, biolistic transformation system, includes helium pressure regulator, solenoid, spacer rods, microcarrier launch assembly, target shelf, 5 macrocarrier holders, tubing
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PDS-1000/He™ System
The PDS-1000/He system accelerates nucleic acid–coated gold or tungsten microparticles (0.6–1.6 µm) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.
The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells
Key Features
- A reproducible method for transforming intact cells in culture, requiring little manipulation of cells
- Transformation of cells with unique growth requirements that are not amenable to other methods of transfection
- Maximum control
- High reproducibility
