Phenylalanine neonate (without Membrane Plate)
High Quality Assays with Reproducible and Reliable Results
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
The Phenylalanine Microwell Enzyme Assay (PHE-MW EA) is designed for the quantitative determination of phenylalanine in neonatal blood spots. It is for use as an aid in screening newborns for elevated phenylalanine levels.The PHE-MW EA is an enzyme assay which follows an acid extraction of the phenylalanine contained in a 3/16” (5 mm) blood spot. In this assay, a single blood spot is extracted in a special semi-porous membrane bottom plate. Following extraction, all 96 extracts are simultaneously transferred to a conventional microwell plate, by means of a vacuum manifold. A neutralizing buffer is added to each acid extract, followed by addition of a combination enzyme-substrate reagent. This rapidly oxidizes the phenylalanine to phenylpyruvate, reducing NAD to NADH+ in the process. The NADH+ reduces a colorless dye to form a colored end-product with an absorbance maximum at 570 nm. The absorbance read is directly proportional to the amount of phenylalanine in the sample.
