Phusion™ High-Fidelity DNA Polymerase
"Finnzymes' unique Phusion™ High-Fidelity DNA Polymerase offers performance that no other enzyme can match. The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a new standard for PCR performance. The error rate…
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Great results
Molecular Biology
Offered with multiple buffers for customized reaction setup for different DNA templates; very efficient
Review Date: 10 Feb 2023 | Finnzymes Oy
High-quality results.
Amplify bacterial DNA
The kit is easy to use and high-quality. The product can be expensive in Latinoamerica but is it worth it to obtain reliable results (PCR products without base changes/deletions)
Review Date: 21 Jan 2022 | Finnzymes Oy
Easy to use and work great!
Amplify DNA fragment from genomic DNA and cDNA
To amplify DNA fragments from genomic DNA and cDNA, Phusion DNA Polymerase was used. It is easy to use and work great. I recommend if you are working for amplification of DNA fragments.
Review Date: 3 Nov 2017 | Finnzymes Oy
probe preparation
Easy to use and good results with herculase, also for amplification in difficult regions.
Review Date: 22 Aug 2014 | Finnzymes Oy
Phusion High-Fidelity Polymerase
This enzyme saved my PhD. Had to amplify and clone roughly 100 constructs and Phusion amplified them all with 100% accuracy. No waste of time searching for the correct clone amplified with Taq or some other so-called "proof-reading" polymerases, just use Phusion and get the correct product on the first attempt.
Review Date: 16 Dec 2013 | Finnzymes Oy
"Finnzymes' unique Phusion™ High-Fidelity DNA Polymerase offers performance that no other enzyme can match. The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a new standard for PCR performance. The error rate of Phusion DNA Polymerase (4.4 x 10-7 when using Phusion™ HF Buffer) is 50-fold lower than that of Thermus aquaticus DNA polymerase, and 6-fold lower than that of Pyrococcus furiosus DNA polymerase - making it the most accurate of available thermostable polymerases.
Incorporating an exciting new technology, Phusion High-Fidelity DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain. By fusing a particular double-stranded DNA binding domain to the polymerase, its processivity can be increased 10-fold. The processivity of Phusion DNA Polymerase is 10-fold greater than that Pyrococcus furiosus DNA polymerase and twice that of Thermus aquaticus DNA polymerase. Phusion High-Fidelity DNA Polymerase exploits this dramatic increase in processivity, resulting in shorter extension times, more robust and high yield amplification, and the ability to amplify long templates in a fraction of the time.
