Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR. It is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.
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Polymerase chain reaction
The DNA Polymerase-High-Fidelity PCR Enzyme is highly effective in accurately replicating DNA sequences with minimal errors. It earns top ratings for ease of use due to its user-friendly protocols and reliable performance. After-sales care typically involves responsive customer support and troubleshooting assistance, further enhancing its appeal. While it may come at a premium price, its exceptional accuracy and minimal need for reagents justify the value for money.
Review Date: 30 Apr 2024 | Thermo Fisher Scientific
Features of Platinum SuperFi II DNA Polymerase include:
- Exceptional >300X Taq fidelity
- Universal primer annealing at 60°C
- Superior specificity, sensitivity, and yields
- Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement)
Platinum SuperFi II DNA Polymerase is an engineered enzyme high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.
Due to the unique composition of the SuperFi II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.
Applications of Platinum SuperFi II DNA Polymerase:
- High-fidelity PCR
- Cloning and sub-cloning
- Site-directed mutagenesis
- Amplification of GC-rich templates
- Template generation for sequencing
- High-throughput PCR
- Amplification of samples with suboptimal purity
- Long PCR
- Fast PCR
For increased convenience, we offer Platinum SuperFi II Master Mix with Platinum SuperFi II DNA Polymerase provided in a ready-to-use mixture along with SuperFi II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. Platinum SuperFi II Green PCR Master Mix is also available, which additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels, further streamlining the PCR workflow from setup to final analysis.
