PREvalence ddPCRTM SARS-CoV-2 Wastewater Quantification Kit

The PREvalence ddPCR^TM SARS-CoV-2 Wastewater Quantification Kit provides reproducible, quantitative results in wastewater testing for SARS-CoV-2, the virus caused by COVID-19.

The PREvalence ddPCR^TM SARS-CoV-2 Wastewater Quantification Kit, based on the U.S. CDC and WHO RT-PCR primers for SARS-CoV-2 detection, allows for the detection of N2 and E genes in SARS-CoV-2. It also includes an assay for the Murine Coronavirus or Murine Hepatitis Virus (MHV), an enveloped, single-stranded RNA virus. MHV serves as a matrix recovery control, enabling estimation of the virus lost during sample processing. The test allows for all three targets to be detected in a single well using the kit. The kit also contains reagents sufficient for 200 reactions, and there is an automated software tool available for analysis.

Features and benefits

• Addresses an unmet need in COVID surveillance — the ability to test millions of people with sufficient lead time to take action
• Quantitative without requiring a standard curve
• More inhibitor-resistant compared to qPCR
• Reproducible results across runs, reagent lots, and instruments
• Single-well multiplex test, or two two-well test if using fecal indicator
• Turnaround time <8 hours from sample to answer
• Validated on the QX200 ddPCR^TM Platforms, compatible with QX ONE ddPCR Platform
• Aligned to CDC guidelines on wastewater testing
• Multiple viral targets for more robust assay performance
• Automatic analysis with the PREvalence Wastewater Automated Analysis Application

Applications and uses

• Detecting SARS-CoV-2 virus in wastewater