PrimeScript™ RT-PCR Kit
The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Excellent kit with high sensitivity and reproducibility.
Analyze clinical blood samples for rare malaria parasite transcripts
Excellent kit with high sensitivity and reproducibility. Annoying having to order through a distributor (I am in Australia). Sometimes the kits have short expiry times which makes things even worse and unfortunately the price is dependent on the strength of the AU$.
Review Date: 5 Oct 2016 | Takara Bio
The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template.
This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enables full-length cDNA synthesis from GC-rich and even highly structured RNA templates. Reverse transcription can be performed at 42°C, avoiding the risk of RNA degradation which may occur at higher temperatures. The PrimeScript RT-PCR Kit generates high yield full-length cDNAs of up to 12 kb in length.
This two-step RT-PCR kit also includes TaKaRa Ex Taq HS for efficient, sensitive and specific amplification of generated cDNA. The combination of PrimeScript with TaKaRa Ex Taq HS results in reliable, efficient and reproducible RT-PCR from a wide variety of RNA templates.
Features:
- RT-PCR kit includes all reagents needed for two-step endpoint RT-PCR (e.g., where products are analyzed using gel electrophoresis)
- Robust reverse transcription and amplification even from challenging RNA templates
- Complete elongation of complex RNA templates
- High yield transcription of full-length cDNA up to 12 kb in length
- Minimal false priming and robust amplification due to the combination of PrimeScript RTase with TaKaRa Ex Taq HS
Applications:
- Two-Step RT-PCR
