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Puromycin-Resistant Mouse Embryonic Fibroblasts, Day E13.5
For generating puromycin-resistant feeder layers for the culture and selection of transfected undifferentiated ES and iPS cells
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MEFs can be used as feeder cells for the maintenance of embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in the undifferentiated state while under selection for a puromycin-resistance marker that has been successfully incorporated into the ES cells or iPS cells. The cells must be mitotically inactivated by irradiation or mitomycin C treatment prior to use as feeder layers. MEFs are resistant to at least 1.5 μg/mL puromycin
