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EvaGreen® Plus Dye, 20X in Water

Biotium#31077, #31077-T

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

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EvaGreen® Plus Dye is an improved alternative to Biotium’s original EvaGreen® Dye. EvaGreen® Plus Dye retains many of the essential benefits of the original patented EvaGreen® Dye, but has an improved signal-to-noise ratio that is more advantageous for a variety of DNA detection applications, including qPCR, digital PCR, and LAMP.

  • Lower background & higher signal compared to original EvaGreen® Dye
  • Better match for common qPCR instrument excitation sources
  • Compatible with qPCR & HRM®
    Higher signal to noise offers advantages for digital PCR and isothermal applications
  • Directly visualize amplification products in gel

Improvements over the Original

Similar to our original EvaGreen® Dye, EvaGreen® Plus Dye is a green fluorescent nucleic acid dye that is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® Plus Dye improves upon these properties with lower background fluorescence and increased brightness upon binding dsDNA. The dye has an excitation maximum that is a better fit for common qPCR instruments, resulting in improved sensitivity. As a result, EvaGreen® Plus Dye can give earlier Ct values and better signal discrimination. These properties offer potential advantages for digital PCR and isothermal amplification applications.

EvaGreen® Plus, 20X in Water, 5x1 mL #31077

EvaGreen® Plus, 20X in Water, 1 mL #31077-T

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