Quick-16S™ Plus NGS Library Prep Kit (V3-V4)
The Quick-16S™ Plus NGS Library Prep Kit (V3-V4) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing.
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Easy Peasy Prep Kit
characterize microbial communities during infection
Great kit to use! It is very straightforward, has all required materials included, and makes prep a breeze. I used it to prep samples for NGS and with no training, I was able to follow the directions included.
Review Date: 6 Dec 2022 | Zymo Research
inexpensive, high quality results
honey bee microbiome
We used this to assess the microbiome of honey bees from healthy hives verses those infested with Varroa mite. It was easy to use and we obtained high quality results.
Review Date: 23 Nov 2022 | Zymo Research
The Quick-16S™ Plus NGS Library Prep Kit (V3-V4) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing.
The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.
Highlights:
- The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
- 100% automation-ready with only a single PCR step and without the need for normalization.
- Real-time PCR enables absolute microbial copy number quantification.
