Quick-RNA Fecal/Soil Microbe Microprep Kit
The Quick-RNA Soil/Fecal Microbe Microprep is an innovative fecal and soil RNA extraction kit designed for the simple, reliable, and rapid isolation of total RNA including small RNAs (>17 nt) from various soil, sludge, sediment and/or fecal samples.
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Reliable RNA extraction kit for soil and similar samples.
Environmental surveillance for SARS-CoV-2
We purchased the Zymo Quick-RNA Fecal/Soil Microbe Microprep Kit after some reference from colleagues that had been working on similar environmental samples (quartz fiber filters). The protocol provided by the manufacturer was overall ok and detailed, although a bit torturous, if compared to similar products. We obtained good results in terms of RNA extraction efficiency (a synthetic RNA Internal Positive Control was added prior to the extraction process) and removal of PCR inhibitors. Actually, we purchased the kit from a local (Italy) dealer and had to wait quite a long time for the product to be shipped.
Review Date: 29 Jul 2021 | Zymo Research
The Quick-RNA Soil/Fecal Microbe Microprep is an innovative fecal and soil RNA extraction kit designed for the simple, reliable, and rapid isolation of total RNA including small RNAs (>17 nt) from various soil, sludge, sediment and/or fecal samples. The procedure of this fecal and soil RNA extraction kit successfully isolates RNA from tough-to-lyse bacteria, fungi, protozoa (protist), algae, etc. in soil, and host RNA from fecal samples. Samples are added to the ZR BashingBead Lysis Tube with an optimally designed S/F RNA Lysis Buffer where microbes are then lysed by bead beating to extract total RNA. The Zymo-Spin column technology included in this fecal and soil RNA extraction kit allows for quick filtration, genomic DNA removal from sample lysates, and isolation of the RNA. Zymo-Spin III-HRC Spin Filter separates RT-PCR inhibitors (e.g., humic acids, polyphenols, tannins) and the total RNA is concentrated using the Zymo-Spin IC Column with a minimum elution volume of ≥6 µl.
