Rapid Transfer Buffer, 10X
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively. Rapid Transfer Buffer is a methanol-free, non-hazardous formulation that is compatible with PVDF and nitrocellulose membranes. It is used for transfer…
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Western Blot
Allows rapid transfer on PVDF or Nitrocellulose membrane without ethanol. 10-15 minutes at 25V is enough for small-medium size proteins.
Review Date: 12 Jun 2017 | AMRESCO (American Research Products)
Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively. Rapid Transfer Buffer is a methanol-free, non-hazardous formulation that is compatible with PVDF and nitrocellulose membranes. It is used for transfer of proteins from standard Laemmli gels, commercial pre-cast gels, and AMRESCO’s NEXT GEL™. The transfer efficiency is equivalent to that observed when using a Tris-Glycine-Methanol transfer buffer.
- Fast, efficient transfer in 10–20 minutes
- Utilizes standard transfer equipment
- Non-hazardous formulation
- Transfers to PVDF and nitrocellulose from multiple types of gels