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Rapid Transfer Buffer, 10X

Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively. Rapid Transfer Buffer is a methanol-free, non-hazardous formulation that is compatible with PVDF and nitrocellulose membranes. It is used for transfer…

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Average Rating 5.0

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Saves time, high quality transfer

 

Average Rating 5.0

Application Area:

Western Blot

Allows rapid transfer on PVDF or Nitrocellulose membrane without ethanol. 10-15 minutes at 25V is enough for small-medium size proteins.

Review Date: 12 Jun 2017 | AMRESCO (American Research Products)

Rapid Transfer Buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively. Rapid Transfer Buffer is a methanol-free, non-hazardous formulation that is compatible with PVDF and nitrocellulose membranes. It is used for transfer of proteins from standard Laemmli gels, commercial pre-cast gels, and AMRESCO’s NEXT GEL™. The transfer efficiency is equivalent to that observed when using a Tris-Glycine-Methanol transfer buffer.

  • Fast, efficient transfer in 10–20 minutes
  • Utilizes standard transfer equipment
  • Non-hazardous formulation
  • Transfers to PVDF and nitrocellulose from multiple types of gels

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