RapiGest™ SF Protein Solubilization Reagent
The patented RapiGest™ SF Surfactant is a reagent used to enhance enzymatic digestion of proteins, both in-gel and in-solution. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin,…
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Very handy stuff!! Yields good results
Proteomics
It really helps to solve a lot of sample prep issues and alongside fosters efficient digestion.
Review Date: 2 Oct 2017 | Waters
A great tool for producing high quality data for complex analyses
Antibody digestion for peptide level analysis via LC-MS
Provides an easy to use option to help produce more complete, reproducible digests.
Review Date: 29 Sept 2017 | Waters
I am going to try it one more time
Digestion of surface proteins
Initial results look promising but I haven't been able to reproduce it.
Review Date: 29 Sept 2017 | Waters
Fantastic tool for N-glycan analysis, sped up our digestion times by 500%!
Rapidly digest mABs for N-glycan analysis
The RapiGest SF Surfactant is an excellent denaturation reagent and cut the total analysis time for our QC Release assay for N-glycan analysis down from 16 hours to only a few hours!
Review Date: 29 Sept 2017 | Waters
RapiGest gives a better digest for select mAbs, but with some non-specific fragments.
Non-reduced solution digestion of mAbs for identity confirmation by peptide mapping
For select mAbs non-reduced, RapiGest produces more complete digestion than digestion in buffer or in combination with an organic solvent. However, expect to observe some undesired non-specific fragmentation.
Review Date: 29 Sept 2017 | Waters
Useful
study of secretome of MSCs
Rapigest definitely is helping to my sample preparation while trypsin digestion. Easy to use and easy to remove after precipitation. I haven't used filtration of my sample since I can clearly remove the precipitate.
Review Date: 29 Sept 2017 | Waters
Easy to use and reproducible results
Analyze biologics of different modalities (mAb, Fusion protein, BiTE, etc) by peptide mapping
The product has been effective in providing the desired results with reduced and non-reduced peptide mapping for different biologic products with reproducible results.
Review Date: 27 Sept 2017 | Waters
Easy to use!
protein sample
Easy to use, saves time, reliable I use it for N-linked Glycan Profiling Analysis of Protein use , using HILIC UPLC.
Review Date: 25 Sept 2017 | Waters
Very effective and easy to use.
Glycan profiling, peptide mapping
The reagent provides very effective denaturation of proteins for peptide map analysis. The protocols are easy to follow. We also use this as part of the RapiFluor MS kit for glycan profiling as a protein denaturant. It is very effective in this regard.
Review Date: 24 Sept 2017 | Waters
Excellent reagent for Glycan profiling
Glycan profiling of mAbs
Very easy to use, yields high quality and consistent results.
Review Date: 22 Sept 2017 | Waters
The patented RapiGest™ SF Surfactant is a reagent used to enhance enzymatic digestion of proteins, both in-gel and in-solution. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.
Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity. It is compatible with enzymes such as Trypsin, Lys-C, Asp-N, Glu-C, PNGaseF and other enzymes.
- Compatible for LC and MS analysis
- Improves efficiency and speed of digestion
- Degraded at low pH and removed from the sample
- Simplifies preparation protocols and improves throughput of analysis
US Patent No: 7,229,539 and US 8,580,533
