Absolute Quantitation of RapiFluor-MS Labeled N-Glycans by Calibration of Fluorescence Response

Glycosylation plays an important role in the structure and function of proteins, and altered glycosylation is implicated in numerous diseases such as cancer, autoimmune diseases and neurodegenerative diseases. In this application note, Waters presents a new method for the preparation and analysis of N-glycans and shows how the RapiFluor-MS Quantitative Standard can be used to calibrate fluorescence response and absolute quantitation.