ReadiDrop™ 7-AAD

Use ReadiDrop 7-AAD (7-aminoactinomycin D), a ready-to-use cell viability dye, for excluding dead cells in flow cytometric experiments. As a cell-impermeable assay, ReadiDrop 7-AAD enters a compromised cell and binds to double-stranded DNA with a high-affinity intercalation between GC base pairs.

The assay removes the traditional manual weighing, pipetting, and dilution steps when using commonly available cell viability dyes in flow cytometry experiments. Simply add 1 or 2 drops of the cell viability assay to assess the health of your cells.

Key Benefits of the ReadiDrop 7-AAD Cell Viability Dye

• Ideal for cell sorting — fluorescent molecules are suspended in PBS without preservatives
• Stable at room temperature — keep a bottle of any ReadiDrop 7-AAD cell viability assay by your cell sorter or cell analyzer for easy access
• No more weighing, pipetting, and diluting — ready-to-use solution gives 1 µg/ml as the final concentration

Features of the ReadiDrop 7-AAD Cell Viability Dye

• Fluorophore Properties
  • Excitation maximum: 548 nm
  • Emission maximum: 648 nm
  • Optimal excitation laser: 561 nm or 488 nm
• Molecular weight: 1270.43 g/mol
• Molecular formula: C₆₂H₈₇N₁₃O₁₆
• Specifications
  • Store at 15–30°C
  • Do not freeze