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Recombinant DNase I (RNase free)

Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on sing…

Thermo Fisher Scientific

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Application Area:

RNA purification

Thermo Scientific™ DNase I, RNase-free is easy to use and gives good and reproducible results.

Review Date: 17 Jul 2021 | Thermo Fisher Scientific

Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on single- and double- stranded DNA, chromatin, and RNA:DNA hybrids. rDNase I can be inactivated by a 10-minute incubation at 75°C.

Applications

  • Remove template DNA after in vitro transcription reactions
  • Nick translation
  • DNase footprinting
  • RNA purification

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