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Recombinant Human Angiotensin-converting Enzyme 2 (ACE2)

Recombinant Human Angiotensin-converting Enzyme 2 (ACE2) with C-terminal His-tag, derived from the transfected human HEK293 cells.

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RayBiotech Inc.

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Product Specifications

Size:

100 ug, 500 ug, or 1000 ug

Format:

Liquid

Formulation:

Supplied as a 0.2 um filtered solution in PBS (pH 7.4)

Purification:

His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)

Concentration:

Lot specific (see the label on the vial), determined by BCA protein assay

Purity:

>95 %

Purity determined by:

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

Recommended Applications:

Binding assay, enzyme activity assay, ELISA, glycosylation analysis, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, etc.

Source

Species:

Human

Accession Number:

Q9BYF1

Expressed Region:

Gln18-Ser740 (Extracellular domain)

Protein Name & Synonyms:

ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15

Preparation

Expression System:

Human embryonic kidney 293 (HEK293) cells

Conjugation/Tag:

C-terminal his-tag

Molecular Weight (kDa):

Recombinant protein product has a calculated molecular mass of ∼83 kDa. Due to the abundant glycosylation, it migrates as approximately 90 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.

SDS-PAGE Image

Stability & Storage

Shipping

Ice packs

Storage/Stability

Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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