Recombinant SARS-CoV-2 Nucleocapsid Protein
Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Specifications
Size:
100 ug, 500 ug, or 1000 ug
Format:
Liquid
Formulation:
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>90%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development.
Source
Species:
SARS-CoV-2
Accession Number:
QHD43423
Expressed Region:
Met1-Ala419
Protein Name & Synonyms:
Nucleocapsid Protein, N Protein
Preparation
Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below.
SDS-PAGE Image
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
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