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Recombinant SARS-CoV-2 Nucleocapsid Protein

Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

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RayBiotech Inc.

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Product Specifications

Size:

100 ug, 500 ug, or 1000 ug

Format:

Liquid

Formulation:

Supplied as a 0.2 um filtered solution in PBS (pH 7.4)

Purification:

His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)

Concentration:

Lot specific (see the label on the vial), determined by BCA protein assay

Purity:

>90%

Purity determined by:

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

Recommended Applications:

Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development.

Source

Species:

SARS-CoV-2

Accession Number:

QHD43423

Expressed Region:

Met1-Ala419

Protein Name & Synonyms:

Nucleocapsid Protein, N Protein

Preparation

Expression System:

Human embryonic kidney 293 (HEK293) cells

Conjugation/Tag:

C-terminal his-tag

Molecular Weight (kDa):

Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below.

SDS-PAGE Image

Stability & Storage

Shipping

Ice packs

Storage/Stability

Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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Spike Protein, S2 Subunit

230-01103

Nucleocapsid Protein

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S1 Subunit Protein (RBD) with C-terminal Fc tag

230-20405

S1 Subunit Protein (RBD) with C-terminal His-tag

230-20406

S1 Subunit Protein with C-terminal His-tag

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S2 Subunit Protein with C-terminal His-tag

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Nucleocapsid Protein with C-terminal His-tag

230-20409

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