Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)
Recombinant SARS-CoV-2, S1 Subunit Protein (RBD) with C-terminal His tag, derived from the transfected human HEK293 cells.
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Product Specifications
Size: 100 ug, 500 ug, or 1000 ug
Format: Liquid
Formulation: Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification: His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration: Lot specific (see the label on the vial), determined by BCA protein assay
Purity: >90 %
Purity determined by: SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications: Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, binding assay, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development.
Sandwich ELISA: this protein functions as a standard in conjunction with 130-10815 (capture antibody) and 130-10814 (detection antibody).
Species: SARS-CoV-2
Accession Number: QHD43416
Expressed Region: Arg319-Phe541
Protein Name & Synonyms: Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)
Preparation
Expression System: Human Embryonic Kidney 293 (HEK293) Cells
Conjugation/Tag: C-terminal his-tag
Molecular Weight (kDa): Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Binding Function
The product S1 RBD (Cat. 230-30162) was coated in 96 well plate and incubated with the serial diluted human ACE2 protein (Raybio, Cat. 230-30165). The bound ACE2 was detected by anti-ACE2 antibody using ELISA. The calculated EC50 is 4.23-9.26 ng/mL.
Stability & Storage
Shipping: Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Related Coronavirus Proteins
Spike Protein, S1 Subunit, Host Cell RBD - 230-01102
Spike Protein, S2 Subunit - 230-01103
Nucleocapsid Protein - 230-01104
S1 Subunit Protein (RBD) with C-terminal Fc tag - 230-20405
S1 Subunit Protein (RBD) with C-terminal His-tag - 230-20406
S1 Subunit Protein with C-terminal His-tag - 230-20407
S2 Subunit Protein with C-terminal His-tag - 230-20408
Nucleocapsid Protein with C-terminal His-tag - 230-20409
