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Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)

Recombinant SARS-CoV-2, S1 Subunit Protein (RBD) with C-terminal His tag, derived from the transfected human HEK293 cells.

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RayBiotech Inc.

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Product Specifications

Size: 100 ug, 500 ug, or 1000 ug

Format: Liquid

Formulation: Supplied as a 0.2 um filtered solution in PBS (pH 7.4)

Purification: His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)

Concentration: Lot specific (see the label on the vial), determined by BCA protein assay

Purity: >90 %

Purity determined by: SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

Recommended Applications: Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, binding assay, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development.

Sandwich ELISA: this protein functions as a standard in conjunction with 130-10815 (capture antibody) and 130-10814 (detection antibody).

Species: SARS-CoV-2

Accession Number: QHD43416

Expressed Region: Arg319-Phe541

Protein Name & Synonyms: Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)

Preparation

Expression System: Human Embryonic Kidney 293 (HEK293) Cells

Conjugation/Tag: C-terminal his-tag

Molecular Weight (kDa): Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.

Binding Function

The product S1 RBD (Cat. 230-30162) was coated in 96 well plate and incubated with the serial diluted human ACE2 protein (Raybio, Cat. 230-30165). The bound ACE2 was detected by anti-ACE2 antibody using ELISA. The calculated EC50 is 4.23-9.26 ng/mL.

Stability & Storage

Shipping: Ice packs

Storage/Stability

Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

Spike Protein, S1 Subunit, Host Cell RBD - 230-01102

Spike Protein, S2 Subunit - 230-01103

Nucleocapsid Protein - 230-01104

S1 Subunit Protein (RBD) with C-terminal Fc tag - 230-20405

S1 Subunit Protein (RBD) with C-terminal His-tag - 230-20406

S1 Subunit Protein with C-terminal His-tag - 230-20407

S2 Subunit Protein with C-terminal His-tag - 230-20408

Nucleocapsid Protein with C-terminal His-tag - 230-20409

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