SageHLS
The SageHLS provides unique electrophoretic method for purifying high molecular weight DNA. Using a cell suspension input, DNA is extracted and immobilized on an agarose surface. This is followed by an enzymatic-mediated fragmentation and automated DNA size selection and collection. The platform features the HLS-CATCH process which employs Cas9 mediated targeting of specific genome targets up to 400kb in length.
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Using a cell suspension input, DNA is extracted and immobilized on an agarose surface. This is followed by an enzymatic-mediated fragmentation and automated DNA size selection and collection. The platform features the HLS-CATCH process which employs Cas9 mediated targeting of specific genome targets up to 400kb in length.
Features include:
- One step sample prep with DNA extraction, enzymatic reaction and DNA size selection occurring in a continuous workflow within an agarose cassette.
- A heated nest allows enzymatic reactions to occur in situ within the agarose cassette.
- Extraction can be performed on a broad range of cell types including cell lines, primary cells and white blood cells.
- Pulse field technology allows purifcation of genomic DNA up to 1MB.
Long-read sequencing
Bar-coded linked reads
Pseudogene, segmental duplication, copy number variant analysis (HLS-CATCH)
