ScreenFect A Transfection Reagent
ScreenFect™ A is a cationic lipid transfection reagent combining high transfection efficiency with low cell toxicity.
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Offering a simple one-step protocol which combines both transfection and plating of cells, ScreenFectA can be used to transfect cells with DNA or siRNA.
Transfection can typically be detected in 24-48h and the low cell toxicity of ScreenFect A eliminates the need to change media post-transfection.
ScreenFect A offers a simple one-step protocol which combines both transfection and plating of cells. Cells from the logarithmic growth phase are detached from their routine culture vessel and suspended in medium. Diluted ScreenFect A and DNA/siRNA complex is then added to the cell suspension and mixed gently by pipetting before transfer to the plate.
Transfection results can typically be detected in 24-48h. The low cell toxicity of ScreenFect A eliminates the need to change media post-transfection and since ScreenFect A can be added directly to media containing serum and antibiotics transfection protocols can be fuss-free.