Products & ReviewLife Sciences
Sigma-Aldrich® CRISPR synthetic gRNAs
Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including microinjection, electroporation, and lipofection.
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Merck's single guide RNAs are purified using gold standard HPLC methods ensuring the highest quality and lowest toxicity in cellular and transgenic applications.
Selected Applications
- Engineer transgenic animal models
- Model disease states in immortalized cells
- Create isogenic iPS cell lines
Advantages
- Guaranteed - Assured cutting for any target sequence including custom designs
- HPLC Purified for unparalleled quality
- Easy-to-use - Reconstitute gRNA and form RNP complexes within minutes
- Customizable – Choose your format, quantity, purification and modification
- Fast shipping (usually 3-5 days for crRNA, 7-10 business days for sgRNA)
- A range of purification and modification options
- Multi-scale delivery in tubes or plates