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SingleShot™ SYBR® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

Bio-Rad1725095

SingleShot™ SYBR® Green One-Step Cell Lysis and RT-qPCR Kit, 100 x 50 µl reactions

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Use the SingleShot™ SYBR® Green One-Step Kit to obtain high-quality RT-qPCR data with SYBR® Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.

Benefits include:

  • Cell lysate from 10 to 100,000 cells ready in 20 minutes
  • Complete removal of genomic DNA while preserving RNA integrity
  • Optimal accuracy and linear dynamic range of qPCR data across genes
  • Results comparable to using purified RNA, including dynamic range
  • Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )
  • SingleShot RNA Control ensures optimal cell and lysate inputs

Optimized Sample Input

The SingleShot™ SYBR® Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:


Kit Contents



Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot™ SYBR® Green One-Step Kit

The SingleShot™ SYBR® Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Related Products

The SingleShot™ SYBR® Green One-Step Kit belongs to the family of SingleShot™ Cell Lysis RT-pPCR Kits :

  • SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )
  • SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )
  • SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )

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