SMARTer® ThruPLEX® DNA-seq
ThruPLEX DNA-seq Kit—General Purpose Library Preparation for Illumina NGS Platforms
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ThruPLEX DNA-seq Kit—General Purpose Library Preparation for Illumina NGS Platforms
ThruPLEX DNA-seq kits build on the innovative ThruPLEX chemistry to generate DNA libraries from as little as 50 pg of DNA and increase sensitivity while providing up to 96 indexes for multiplexing. These kits can be used with fragmented DNA from any sample source—genomic DNA, biofluids such as cell-free DNA, DNA from FFPE materials, and cDNA. The entire ThruPLEX DNA-seq kit workflow is performed in a single tube or well in about 2 hours and requires no purification steps or sample transfers. Kits contain 12, 48, or 96 single- or dual-read Illumina-compatible indexes either in tubes (6 or 12) or pre-dispensed and sealed in barcoded microplates. The ThruPLEX DNA-seq kits can be used in any DNA-seq application, RNA-seq, or ChIP-seq and offer robust target enrichment performance with all of the leading platforms.
Features:
- High performance from lowest input—50 pg to 50 ng
- Complete package for creating libraries—optimized indexing and adapter reagents, enzymes, buffers, and nuclease-free water
- Fast and simple workflow—three steps in a single tube or well in about 2 hours, no transfers necessary
- Compatible with major target enrichment platforms—Agilent SureSelect, Roche Nimblegen SeqCap EZ, and IDT xGen Lockdown probes
