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SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml

Bio-Rad1725160

1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 µl reactions

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Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.

  • Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
  • Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
  • For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays , self-designed assays, and TaqMan Assays

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* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.

Simple Workflow

Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.

Recommended RT-qPCR Reagents and Assays

Reverse Transcription

  • iScript™ Reverse Transcription Supermix for RT-qPCR
  • iScript™ Advanced cDNA Synthesis Kit for RT-qPCR

qPCR Supermixes

  • SsoAdvanced™ Universal SYBR® Green Supermix
  • SsoAdvanced™ Universal Probes Supermix

PreAmp and qPCR Assays

  • PrimePCR™ PreAmp Assays
  • PrimePCR™ Assays

More Information

Find out more about the features of the SsoAdvanced™ PreAmp Supermix .

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