SsoAdvanced™ PreAmp Supermix, 50 x 50 µl rxns, 1.25 ml
1 ml (1 x 1 ml vials), contains antibody-mediated hot-start Sso7d-based DNA polymerase (patented), dNTPs, salts, enhancers, stabilizers, for 50 x 50 µl reactions
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Use the SsoAdvanced PreAmp Supermix to achieve the unbiased preamplification of up to 100 qPCR target sequences in a single reaction.
- Start with as little as 100 pg of cDNA — amplify precious samples like stem cells, laser capture microdissection (LCM), and formalin-fixed paraffin-embedded tissues (FFPE)
- Compatible with PrimePCR™ PreAmp Assays, custom-designed assays, and TaqMan Gene Expression Assays
- For gene expression and genotyping applications — validated with PrimePCR™ PreAmp Assays , self-designed assays, and TaqMan Assays
Product Comparison
* Relative quantification between nonamplified and preamplified cDNA.
** As of May 2014.
Simple Workflow
Setting up a preamplification takes only a few minutes. Simply combine the pooled assays, SsoAdvanced PreAmp Supermix, and template in a microcentrifuge tube, and start the PCR reaction. In less than 2 hours, an unbiased 1,000-fold amplification of starting template is complete and ready for downstream analyses.
Recommended RT-qPCR Reagents and Assays
Reverse Transcription
- iScript™ Reverse Transcription Supermix for RT-qPCR
- iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
qPCR Supermixes
- SsoAdvanced™ Universal SYBR® Green Supermix
- SsoAdvanced™ Universal Probes Supermix
PreAmp and qPCR Assays
- PrimePCR™ PreAmp Assays
- PrimePCR™ Assays
More Information
Find out more about the features of the SsoAdvanced™ PreAmp Supermix .
