SsoAdvanced™ Universal SYBR® Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, ROX normalization dyes, for 200 x 20 µl reactions
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
It is good.
qPCR
It is very easy to use and technical support is good.
Review Date: 25 May 2021 | Bio-Rad
For Real Time PCR
We use this product for Real Time PCR Assay done on BioRad CFX96. This product was suggested to us by our local BioRad manager and I am glad we tried it. It is easy to use, turns out to be economical and results have been consistent and quick. I would definitely recommend this product. I keep hearing about how if you are not using Taqman, you are not getting the right results, it is not true anymore. Welcome to the future!
Review Date: 23 Jan 2015 | Bio-Rad
SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX dependent or independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.
SsoAdvanced Universal SYBR Green Supermix lets you:
- Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
- Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
- Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
- Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
- Obtain better results with Bio-Rad's PrimePCR™ assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance
Instrument Compatibility
SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:
* BSA must be added according to instrument specifications
Applications and Uses of SsoAdvanced Universal SYBR Green Supermix
- qPCR/real-time PCR
- Gene expression analysis
- Pathway analysis
- Absolute quantification
- Chromatin immunoprecipitation (ChIP) qPCR
- Mutation detection
- Pathogen detection
- Viral detection (load)
- Characterization of genetically modified organisms (GMO)
- Genetic profiling
Related Products
We have developed SsoAdvanced universal probes supermix , which is available in several formats (200–5,000 reactions).
Bio-Rad offers a complete workflow solution for real-time PCR, including:
- RNA isolation kits
- Reverse transcription kits
- Real-time PCR supermixes
More Information
To ensure that your qPCR reactions are reproducible and reliable, follow our step-by-step design and optimization protocols . We also provide insights into analysis of qPCR data and troubleshooting reactions .
* U.S. patents 6,627,424; 7,541,170; and 7,560,260.
