StarBright™ Blue 700 Fluorescent Secondary Antibodies
The StarBright Blue 700 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 470 nm and emission maximum at 705 nm. StarBright Blue 700 is a brighter alternative to PerCP-Cy5.5, with less spillover, and does not exhibit tandem breakage.
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StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.
StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc MP Imaging System, permitting detection of up to three proteins in a single blot.
Schematic representation of a StarBright Blue 700 Label conjugated to an antibody. The light-absorbing monomer is excited at 470 nm and, via an efficient intramolecular transfer process, the energy is transmitted to a light-emitting monomer, which emits at 700 nm. An antibody is conjugated to linker monomers.
Features and Benefits
- Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot
- Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
- High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm)
- Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies
Applications and Uses
StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores as described in the table below. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
Note: StarBright Antibodies are not suitable for stripping and reprobing.
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Products Model Information
StarBright™ Blue 700 Goat Anti-Mouse IgG, 80 µl
Bio-RadPkg of 1, 80 µl tube of lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 fluorophore
StarBright™ Blue 700 Goat Anti-Mouse IgG, 400 µl
Bio-RadPkg of 1, 400 µl tube of lyophilized goat anti-mouse IgG conjugated with StarBright Blue 700 Fluorophore
StarBright™ Blue 700 Goat Anti-Rabbit IgG, 80 µl
Bio-RadPkg of 1, 80 µl tube of lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 fluorophore
StarBright™ Blue 700 Goat Anti-Rabbit IgG, 400 µl
Bio-RadPkg of 1, 400 µl tube of lyophilized goat anti-rabbit IgG conjugated with StarBright Blue 700 fluorophore