Tag-ChIP-IT®
Perform ChIP without the need for protein-specific antibodies
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,b>Active Motif’s Tag-ChIP-IT® Kit is designed to enable chromatin immunoprecipitation of transcription factors, sequence variants and truncated proteins that have been expressed using Active Motif’s unique AM-tag.
The AM-tag is specifically designed to minimize cross-reactivity with mammalian samples, thereby reducing background signal. Another benefit of the AM-tag is that the sequence is unstructured which allows the tag to protrude from the protein of interest for maximum exposure during the immunoprecipitation. This increases enrichment efficiency by allowing even low abundance transcription factors to be detected. Simply clone the AM-tag in frame with the C-terminus of your protein of interest in the expression vector of your choice. Following transfection and expression of your tagged protein, the Tag-ChIP-IT Kit can be used to isolate chromatin and perform immunoprecipitation using an antibody directed against the AM-tag.
