Taq DNA Polymerase from Thermus aquaticus
with 10× PCR reaction buffer without MgCl 2
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One of cheaper and infallibile that I've used
MOlecular BIOLOGY
Taq DNA polymerase comes with the possibility to choice beetween a 10× reaction buffer including opti mixer concentration of MgCl2 USEFUL for routine PCR or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration. The latter option is VERY USEFUL to research purpose when IT is necessary to determine optimal conditions for amplification.
Review Date: 17 Jan 2019 | Merck
Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.
