TB Green Premix Ex Taq II (Tli RNase H Plus)

This Takara Bio SYBR Green Master Mix is recommended for real-time PCR (qPCR) when high specificity is critical, because this reagent effectively suppresses amplification of primer-dimers and off-target products.

Use this product when primer design has been optimized, but an increase in specificity of the qPCR assay is still needed. SYBR Premix Ex Taq II (Tli RNase H Plus) includes SYBR Green I, a reagent designed for intercalator-based qPCR. Additionally, SYBR Premix Ex Taq II (Tli RNase H Plus) is supplied at a 2X concentration, enabling easy reaction assembly. The premix also contains heat-resistant Tli RNase H Plus, which effectively degrades residual mRNA found in the input cDNA, thereby minimizing qPCR inhibition as a result of residual mRNA presence.

Takara SYBR Green Master Mix products contain TaKaRa Ex Taq HS DNA Polymerase, a hot start PCR enzyme that includes an anti-Taq antibody. The use of an anti-Taq antibody allows highly reproducible and reliable qPCR. SYBR Premix Ex Taq II (Tli RNase H Plus) also enables high reaction specificity and superior performance with GC-rich templates.


Features:

• High specificity minimizes primer-dimer formation and nonspecific amplification
• Excels with GC-rich targets
• 2X master mix provides convenience
• High sensitivity: detects fewer than 100 copies
• Compatible with all commonly used qPCR instruments
• Includes SYBR Green Master Mix for intercalator-based qPCR
• Tli RNase H Plus reduces inhibition from mRNA/cDNA hybrids in qPCR
• Eliminates need for separate RNase H digestion step when using low RNase H RTs

Applications:

• Gene expression analysis
• qPCR
• Genotyping
• Suppression of primer-dimers or off-target products