TGF-B1 ELISA
High Quality Assays with Reproducible and Reliable Results
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Testing for food and beverage
Food and Beverage
In general, the kit is good. However, the kit lacks testing instructions for food or beverage testing. The kit also gets low recovery (70-80%) on samples with high protein contents.
Review Date: 12 Nov 2018 | DRG International Inc.
An enzyme immunoassay for the quantitative measurement of TGF-Beta1 in serum and cell culture supernatant. Transforming Growth Factor Beta1 (TGF-Beta1) is a 25 kDa Homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-Beta1 is a multipotent Cytokine with cell- and dose-dependent activities. This molecule is produced by a number of cells and tissue types, e.g. thrombocytes, bone tissue, placenta and kidneys. This potent Cytokine modulates embryonic development, bone formation, mammary development, wound healing, hematopoiesis, cell cycle progression and the production of the extracellular matrix. With respect to the immune system, TGF-Beta1 inhibits T and B cell proliferation and acts as an anti-inflammatory molecule both in vitro and in vivo. TGF-Beta1 inhibits macrophage maturation and activation. This molecule also inhibits the activity of natural killer cells and lymphokine activated killer cells and blocks cytokine production.The DRG TGF-Beta1 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Prior to testing the standards and samples are diluted in assay buffer, acidified with HCl and then neutralized with Neutralization Buffer. Thereafter, the neutralized standards and samples are added to the antibody coated (polyclonal) microtiter wells. After incubation unbound sample material is removed by washing. In a second step monoclonal mouse anti TGF-Beta1 antibody, a biotinylated anti mouse IgG antibody and the Streptavidin-HRP Enzyme complex are incubated successively, forming an immuno enzyme sandwich complex. After incubation the unbound conjugate is washed off. Having added the substrate solution, the intensity of color developed is proportional to the concentration of TGF Beta1 in the sample.
