Products & ReviewLife Sciences

Thermo Scientific Extensor Long Range PCR Polymerase Blend-Kits and Master Mixes

Extensor Long Range Enzyme Blend is an optimized mix of ThermoPrime* DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20kb. This makes the enzyme blend ideal for robust full-length amplification of long range sequences. ■ Amplifies targets up to 20kb in length ■ Unique enzyme blend and optimized buffer combination delivers increased accu…

Thermo Fisher Scientific

The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our  Product Directory.

Average Rating 4.6

|6Scientists have reviewed this product

Ease of Use
After Sales Service
Value for Money
Write your own review

Great results, High-Fidelity, easy to use, saves time!!

 

Average Rating 5.0

Application Area:

To analyze DNA sequence of interest (reverse genetics)

This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps. It helps to set up controls and test different concentrations of their target DNA or RNA templates without having to individually add precise amounts of enzymes, buffers, cofactor, water and dNTP to each reaction tube or plate well. Due to a reduced number of pipetting steps required for PCR set up, it becomes easier to work in the lab. I can say this product is cost-effective in comparison to its usage as it is feasible and effective in minimal volumes. High-Fidelity, easy to use, save time.

Review Date: 4 Oct 2023 | Thermo Fisher Scientific

High fidelity and accuracy Easy to use Reliable Available in a variety of formulations

 

Average Rating 4.0

Application Area:

Pathogen detection

I have used the Thermo Scientific Extensor Long Range PCR Polymerase Blend-Kits and Master Mixes on a number of occasions and have been very impressed with the results. I have been able to amplify long DNA fragments with high fidelity and accuracy. The kits and master mixes are easy to use and have been reliable. I would highly recommend them to anyone who needs to amplify long DNA fragments.

Review Date: 19 May 2023 | Thermo Fisher Scientific

It allowed to obtain reproducible and acute results

 

Average Rating 5.0

Application Area:

Gene expression

Easy to use, I got acute and reproducible results

Review Date: 28 Nov 2022 | Thermo Fisher Scientific

Good for laboratory testing

 

Average Rating 4.3

Application Area:

Plant pathologen detection

We found kit good sensitive, specific detection and quantification of nucleic acid targets.

Review Date: 19 Nov 2021 | Thermo Fisher Scientific

Good for laboratory testing

 

Average Rating 4.3

Application Area:

Plant pathologen detection

We found kit good sensitive, specific detection and quantification of nucleic acid targets.

Review Date: 19 Nov 2021 | Thermo Fisher Scientific

Great results

 

Average Rating 5.0

Application Area:

Biomedical research

One of the best product in the market in terms of money and results and ease of use

Review Date: 27 Apr 2018 | Thermo Fisher Scientific

Extensor Long Range Enzyme Blend is an optimized mix of ThermoPrime* DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20kb. This makes the enzyme blend ideal for robust full-length amplification of long range sequences.

■ Amplifies targets up to 20kb in length
■ Unique enzyme blend and optimized buffer combination delivers increased accuracy and yield
■ PCR products generated can be used directly in T/A cloning
■ Optimized buffering systems for success with a variety of templates
■ Buffer 1 for success with most templates up to 12kb in length
■ Buffer 2 for templates 12-20kb and GC-rich or other difficult templates of any length

Extensor is available in a range of formats and pack sizes to suit any workflow.

Format Options:
■ Enzyme Kits are the most basic format. Kits include the enzyme, PCR buffers 1 and 2, and 25mM MgCl2 solution, each in separate vials. dNTPs must be purchased separately
■ Complete PCR Kits include all the components contained in the Enzyme Kit, plus they have HPLC-purified dNTP mix. Complete PCR kits are a convenient option that provides all the components necessary for PCR
■ Master Mix formats consist of a single master buffer containing all the components needed for PCR. Master Mixes are the most convenient format and significantly reduce assay set-up steps
■ ReddyMix Master Mix formats consist of a single ReddyMix master buffer containing all the components needed for PCR. ReddyMix master mix can be directly loaded onto agarose gels post-PCR
■ PCR Buffers: All kits are supplied with both Buffer 1 and Buffer 2 to accommodate a variety of template types. Master mixes come in either Buffer 1 or Buffer 2 formulation

Storage Conditions: Store at -20°C. Avoid freeze-thaw cycles.

Recommended for: Amplifying fragments up to 20kb; PCR; Cloning; Long PCR

Product Overview

Links