Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit
Prepare concentrated nuclear extracts for an EMSA and gel shift assays. The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein f…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Great result.
Molecular biology
It's an easy to use kit. Applicable to research in gram positive and negative bacteria.
Review Date: 12 Oct 2021 | Thermo Fisher Scientific
Straight forward, good results.
Protein fractionation
This kit is quite fast and easy to use, but nuclear pellet lysis efficiency depends heavily on the kind of cells used (some require more incubation time and syringe lysis before the final centrifugation step). Nuclear and cytoplasmic fractions are quite pure according to Lamin A-tubulin levels.
Review Date: 12 Dec 2020 | Thermo Fisher Scientific
Very easy to use and good results and yeild of DNA
Used to extract plasmids for use in DNA transformation
The prep kit was very easy to use and fairly inexpensive. The students were able to get good results and yield of DNA and help the experiment move much faster
Review Date: 3 Jun 2019 | Thermo Fisher Scientific
Nuclear and Cytosolic protein isolation from Drosophila tissues and cell lines
This extraction kit works really well. The step by step instructions are really easy to follow and perform. We get very good yield and purity of nuclear and cytoplasmic fractions.
Review Date: 22 Dec 2014 | Thermo Fisher Scientific
Prepare concentrated nuclear extracts for an EMSA and gel shift assays.
The Thermo Scientific NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.
The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
Features:
- Fast – obtain nuclear and cytoplasmic fractions of soluble proteins in less than two hours
- Proven – the NE-PER Reagent Kit is referenced in more than 950 distinct publications
- Versatile – nuclear protein from either cultured cells or tissues (intended for fresh samples only)
- Scalable – two kit sizes for producing extracts from cells and tissues
- Convenient – simple instructions do not require ultracentrifugation over gradients
- Compatible – use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays
