Thermo Scientific T4 DNA Polymerase
Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity. Highlights • Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1) • Active in Therm…
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Thermo ScientificT4 DNA Polymerase, a template-dependent DNA polymerase, catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.
Highlights
• Stronger 3'-5' exonuclease activity on single-stranded than on double-stranded DNA and greater (more than 200 times) than DNA polymerase I, E. coli,and Klenow fragment (see Reference1)
• Active in Thermo Scientific restriction enzyme, PCR, RT and T4 DNA Ligase buffers
Applications
• Blunting of DNA ends: fill-in of 5'-overhangs or/and removal of 3'-overhangs
• Blunting of PCR products with 3'-dA overhangs
• Synthesis of labeled DNA probes by the replacement reaction
• Oligonucleotide-directed site-specific mutagenesis
• Ligation-independent cloning of PCR products
