Thermo Scientific Thermo-Start Taq DNA Polymerase & Master Mixes

Thermo-Start Taq DNA Polymerase is a hot-start enzyme combined with an optimized buffer system that gives superior specificity and sensitivity. The chemically modified Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction. This prevents nonspecific amplification during reaction setup, eliminating smearing and the formation of nontarget bands.

■ Hot-Start modification ensures stringent specificity
■ Safe for room temperature reaction setup
■ Enhanced enzyme stability for increased yield and sensitivity
■ Advanced buffering system minimizes PCR optimization steps
■ High Performance Buffer formulation for GC-rich and other complex templates

Thermo-Start is available in a range of formats and pack sizes to suit any workflow.
Format Options:

■ Enzyme Kits are the most basic format. Kits include the enzyme, PCR buffer, and 25mM MgCl2 solution, each in separate vials. dNTPs must be purchased separately.
■ Complete PCR Kits include all the components needed to perform PCR, including an HPLC-purified dNTP mix. Complete PCR kits are a convenient option that still allows for MgCl2 optimization.
■ Master Mix formats consist of a single master buffer containing all the components needed for PCR. Master Mixes are the most convenient format as they significantly reduce assay set-up steps and minimize the risk of sample contamination.
■ Premixed MgCl2 Kits have MgCl2 premixed into the PCR buffer and will be at 1.5mM concentration in the final reaction.
■ High Performance Buffer Kits are specially formulated for GC-rich or other complex targets with a high degree of secondary structure.

Storage Conditions: Store at -20°C for long-term storage. Stable at +4°C for up to four weeks. Avoid freeze-thaw cycles.

Compatible with: Thermo-Start Taq Reddymix Kits and Master Mixes