Thermo Scientific™ Amino Acid Standard H
Thermo Scientific Pierce Amino Acid Standard H is a quantitative mixture of 18 amino acids, supplied at 2.5 µMol/mL each in 0.1N HCl, for use as a high-purity calibration standard for HPLC analysis of protein hydrolysates.
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The best standard for quality control of amino acids
Quality control, amino acid analysis
The most important thing of all is that it is exactly the same reagent that waters sells. Literally because waters takes this reagent, changes the labels and sells them with their own. It can be checked since the COAs are from Thermo itself. The reagent works quite well in order to perform quality controls in the same sequence. However, we have the theory that the analysis certificate offered by the seller is not very accurate, but it is the best that is currently on the market.
Review Date: 7 Dec 2022 | Thermo Fisher Scientific
Product features:
- Complete mixture—contains eighteen (18) amino acids for assessment of amino acid composition of peptide hydrolysates
- Quantitative—contains exact amounts of each amino acid (2.5±0.1 µMol/mL, except cystine at 1.25±0.1 µMol/mL), enabling its use as a quantitation standard
- Stable—dissolved in 0.1N hydrochloric acid and stabilized as 1 mL aliquots in sealed glass ampules
- Standard H—designed for compositional amino acid analysis by HPLC methods of protein or peptide hydrolysates
This quantitative solution of eighteen purified amino acids in 0.1N HCl is an ideal reference standard for calibrating amino acid analyzers and assessing the composition of hydrolyzed peptides and proteins (hydrolysates). To permit standardization of microbiological and other assays, the mixture uses only the L-form of each amino acid. The molar concentration of each amino acid is verified by conventional amino acid analysis methods, making the mixture suitable as both a qualitative and quantitative standard. The solution is provided as stable 1 mL aliquots in sealed glass ampules.
Product details:
When kept frozen, an unopened vial has an indefinite storage life. Once the seal is broken, the reagent has a maximum storage life of six months (store frozen between uses). To use the product, thaw a vial and shake well to ensure a homogeneous solution. Then dilute appropriately with suitable buffers to a concentration compatible with the full-scale sensitivity of your amino acid analyzer.
