Thermo Scientific™ Mem-PER™ Plus Membrane Protein Extraction Kit
Mem-PER Plus Membrane Protein Extraction Kit enables small-scale solubilization and enrichment of integral and membrane-associated proteins using a simple reagent-based procedure.
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The Mem-PER Plus Kit effectively isolates integral and attached membrane proteins from cultured cells as well as hard and soft mammalian tissues using a simple benchtop microcentrifuge procedure. Integral membrane proteins having one or two membrane-spanning domains are effectively solubilized and isolated from cytosolic proteins. The fractions are directly compatible with many downstream applications such as SDS-PAGE, Western blotting, BCA, immunoprecipitation, and amine-reactive protein labeling techniques
Features:
- Purity—produces minimal cross-contamination (typically less than 10%) of cytosolic protein into the membrane protein fraction
- Versatile—effective for extraction from cultured mammalian cells and mammalian tissues
- Downstream compatibility—analyze membrane protein extracts by SDS-PAGE, western blotting, immunoprecipitation and protein assays
- Fast and simple—isolation of membrane proteins in approximately one hour
- No special equipment required—only a benchtop microcentrifuge, tubes, homogenizers, and pipettors are needed
