Tks Gflex™ DNA Polymerase
Components : Tks Gflex DNA Polymerase 250 U 2 X Gflex PCR Buffer (Mg2+, dNTP plus) 1 ml x 5 Mg2+ conc. (2 X) 2 mM dNTP conc. (2 X) ea. 400 μM
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Should try.
PCR enzyme
Compared to old kits, this has less reagents to add and needs shorter time for extension, thus reducing experiment time. There are very few extra bands as well.
Review Date: 22 Oct 2021 | Takara Bio
Tks Gflex DNA Polymerase is a PCR enzyme improved based on a high fidelity DNA polymerase derived from Thermococcus sp. This enzyme
possesses high fidelity of a family B DNA polymerase and an excellent
extension activity by suppressing its non-specific binding to template
DNA. The Takara Bio’s original strong elongation factor and the newly
developed accelerator for specific priming (patent pending) contribute to high-speed amplification with extremely high specificity from templates in a wide range of nucleic acid concentrations. Therefore this polymerase makes PCR amplification succeed in most cases with such targets much longer and that contain GC- or AT-rich sequences difficult to amplify by general DNA polymerases.
The elements which absorb the substances inhibiting PCR or accelerate amplification in the reaction buffer enable PCR using crude samples to be in high efficiency.
This polymerase is for Hot Start PCR utilizing a monoclonal antibody which suppresses DNA polymerase activity and 3’ -5’ exonuclease activity at room temperature.
