Transcreener® UDP² Glycosyltransferase Assays
The Transcreener® UDP² Glycosyltransferase Assay Kit directly detects UDP—the conserved product of glycosyltransferase reactions—in a homogeneous mix-and-read format. Compatible with FP, FI, and TR‑FRET readouts, it supports UDP concentrations from 1–250 µM and delivers exceptional HTS performance (Z’ ≥ 0.7) across 96-, 384-, and 1536‑well formats.
FP Glycosyltransferase Assay
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Nice product. I was able to establish a robust HTS assay with relative ease.
High throughput screening assay
Through the process of developing and validating an assay for HTS applications, I generated a very nice data package of robust high quality data.
Review Date: 6 Aug 2019 | BellBrook Labs
A universal, mix-and-read assay for direct fluorescent detection of UDP, the invariant product of glycosyltransferase activity. Designed for HTS, it simplifies workflows while ensuring accurate, reproducible results.
Key Features & Benefits
- Direct UDP Detection – No coupling enzymes required; minimizes false positives.
- Universal Application – Works across glycosyltransferases such as galactosyltransferases, glucuronyltransferases, and UGTs.
- Multiple Detection Modes – Supports FP, FI, and TR-FRET readouts for flexibility.
- High Sensitivity – Detects UDP from 1 to 250 μM with robust Z’ ≥ 0.7.
- HTS-Ready – Validated in 96-, 384-, and 1536-well plate formats.
- Automation-Compatible – Stable signal and homogeneous format for streamlined screening.
Applications
- High-throughput screening of glycosyltransferase inhibitors
- SAR and selectivity profiling
- Substrate specificity and mechanistic studies
