Transcriptor First Strand cDNA Synthesis Kit

The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications:

• Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler^® Carousel-Based System, the LightCycler^® 480 System, or other real-time instruments.
• Generating cDNA libraries with large and full-length inserts.
• Cloning genes of interest.
  

The kit contains all components required for cDNA reactions for use with conventional thermal cyclers and real-time PCR instruments. In addition, the 50-reaction pack size includes 10 control reactions.

Benefits

Optimized for Many Applications

• Use with conventional thermal cyclers and real-time PCR instruments.
• Optimize real-time PCR – the kit is tested with the LightCycler^® Carousel-Based System, the LightCycler^® 480 System, and other real-time instruments.
• Obtain accurate linear quantification over at least a 10⁸-fold range of input RNA (in vitro transcripts), permitting the analysis of genes with very low or extremely high expression levels.
• Generate efficient qPCR curves, with high fluorescence intensity and identical distances between RNA dilutions, allowing a straightforward analysis of results.
• Obtain reliable and sensitive results – there are no additives in the RT buffer system that interfere with – or inhibit – the subsequent PCR.

Robustness

• Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA with high secondary structures).
• Achieve high reproducibility.
• Generate full-length transcripts with the anchored-oligo(dT)18 primer that is included in the kit.
• Obtain cDNA transcripts up to 14 kb.

Flexibility

• Use three different priming methods – random hexamers, anchored-oligo(dT)18, and sequence-specific primers – depending on the type of analysis needed.