Transcriptor Reverse Transcriptase

Transcriptor Reverse Transcriptase is designed to transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources, using conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler^® Instruments) for the following applications:

• Synthesis of first-strand cDNA for use in subsequent amplification reactions
• RT-PCR of GC-rich RNA templates
• Cy3, Cy5, DIG, biotin, and aminoallyl labeling during cDNA synthesis
• Retrieving and cloning the 5' and 3' termini of mRNA by RACE
• Generation of cDNA libraries with large inserts
• Dideoxy DNA sequencing
• RNA sequencing
• 3'-end labeling of DNA fragments
• Generation of single-stranded probes for genomic footprints

Benefits

• Achieve high sensitivity in two-step RT-PCR.
  Transcriptor Reverse Transcriptase is used in conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler^® Instruments).
• Obtain more full-length transcripts - up to 14 kb.
  cDNA libraries with large inserts can be generated.
• Reverse transcribe difficult templates.
  The enzyme works well at elevated temperatures, thereby overcoming RNA secondary structure (e.g., GC-rich RNA templates) and facilitating optimal reaction conditions.
• Efficiently label cDNA.
  Cy3-, Cy5-, DIG-, biotin-, or aminoallyl-labeled nucleotides are incorporated during cDNA synthesis.