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TriPure Isolation Reagent

Ready-to-use reagent for the simultaneous isolation of total RNA, DNA, and proteins from cells or tissues of human, plant, yeast, bacterial, or viral origin. RNA is undegraded and free of protein or DNA contamination. Product Description A260/280 ratio: 1.7 – 2.0 DNA analysis: Agarose-gel electrophoresis visualization of undigested DNA predominantly appears as two bands of approximately 20 kb and 60 – 100 kb in length. Restric…

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Ready-to-use reagent for the simultaneous isolation of total RNA, DNA, and proteins from cells or tissues of human, plant, yeast, bacterial, or viral origin. RNA is undegraded and free of protein or DNA contamination.

Product Description

A260/280 ratio: 1.7 – 2.0

DNA analysis:
Agarose-gel electrophoresis visualization of undigested DNA predominantly appears as two bands of approximately 20 kb and 60 – 100 kb in length. Restriction-enzyme digest DNA incubation with Eco RI (4 U/µg DNA) for 18 h at 37°C results in visibly digested DNA.

RNA analysis:
Formaldehyde-agarose gel visualization of RNA isolated from rat shows two intact ribosomal bands. Northern blot analysis visualizes intact β-actin mRNA.

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