TRIzol
TRIzol is a reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
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Flexible product and easy to use
Molecular Biology
Diverse and highly effective reagent in molecular biology. Single product that can address nucleic acid and amino acid extraction from plant tissues.
Review Date: 5 Dec 2022 | Thermo Fisher Scientific
Best product for RNA extraction.
RNA extraction
Have tried many kits for RNA extraction but none of them come close to TRIzol in terms of ease of use, yield and quality of RNA.
Review Date: 15 Jul 2022 | Thermo Fisher Scientific
Excellent product, reproducible results
Total RNA Isolation
Very easy to use. Complete extraction of total RNA, including small RNA with great yields and purity, from cell lines and tissues.
Review Date: 23 May 2022 | Thermo Fisher Scientific
Great product
RNA extraction
An easy and effective way to isolate RNA.
Review Date: 11 Feb 2022 | Thermo Fisher Scientific
I use up a lot of TRIzol reagent and it is my preferred method for obtaining pure RNA
RNA extraction.
RNA yields are even better than the kits. The purity is very high if properly extracted.
Review Date: 21 Jun 2021 | Thermo Fisher Scientific
Description
TRIzol Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples. This monophasic solution of phenol and guanidine isothiocyanate is designed to isolate separate fractions of RNA, DNA, and proteins from cell and tissue samples of human, animal, plant, yeast, or bacterial origin, within one hour.
Key features of TRIzol Reagent include:
• Permits the isolation of RNA, DNA, and protein from the same sample
• Offers superior lysis capability, even with difficult sample types
• Optimized formulations and protocols for tissues, cells, serum, virus, and bacteria
Reliably purify RNA from multiple sample volumes and sources
TRIzol® Reagent performs well with small quantities of tissue (50–100 mg) and cells (5 × 106), as well as with large quantities of tissue (≥1 g) and cells (>107), and comes with prototcols for purification from samples of human, animal, plant, or bacterial origin. TRIzol Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. The simplicity of the TRIzol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in 1 hour. Total RNA isolated by TRIzol Reagent is free of protein and DNA contamination.
Formulated for isolation of multiple molecular targets
TRIzol Reagent allows you to perform sequential precipitation of RNA, DNA, and proteins from a single sample. After homogenizing the sample with TRIzol Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), and interphase and red lower organic layers (containing the DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA is precipitated from the interphase/organic layer with ethanol. Protein is precipitated from the phenol-ethanol supernatant by isopropanol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities, and then resuspended for use in downstream applications.
