Twist Comprehensive Viral Research Panel (2, 12, or 96 reactions)
The Twist Comprehensive Viral Research Panel covers reference sequences for 3,153 viruses, including 15,488 different strains.
Measuring viruses in tissue
We have been using this viral capture panel for about 1 year in our lab. We have been testing against tissues with known viruses, controls and unknowns and so far it has performed incredibly well in FFPE. The reagent cost is extremely reasonable and addition of UDI primers allows for pooling to decrease cost of sequencing. Establishing the protocol to use these reagents was a bit difficult to begin as the protocols are vague to allow for multiple library prep/sequencing options. However, TWIST technical support was very knowledgeable and friendly as we worked through optimizing the best method for our lab. We will continue to use TWIST reagents for the foreseeable future because they are great to work with and the results are reproducible.
Review Date: 21 Jun 2022 | Twist Bioscience
KEY BENEFITS
- Screen for over 3,150 viral human pathogens in a single sample.
- Sequences compiled from RefSeq; known to affect humans
- Increase accuracy and sensitivity of detection NGS-based quality control of the probe library ensures high uniformity
- High capture efficiency for sensitive, specific NGS-based identification
- Circumvents the problem of low levels of virus within high levels of host genetic material
- Save time and money, eliminates the need for costly, timeconsuming PCR-based amplification or transcriptome-based sequencing approaches
- Expedites diagnosis and simplifies tracking of emerging viral infections