Twist Library Preparation EF 2.0 Kit (Enzymatic Fragmentation)
Twist Library Preparation Enzymatic Fragmentation (EF) Kit 2.0 is built to help you achieve a more efficient sequencing pipeline and obtain more accurate Next-Generation Sequencing (NGS) results. This updated kit has new fragmentation and ligation modules, and includes the Equinox Library Prep Amp Mix. This hot-start enzyme formulation has a lower error rate* and high efficiency at low input volumes—making it ideal for samples…
Next Generation Sequencing
The library production kit was super easy to use and produced high quality fungal genomic DNA libraries straight out of the box with no optimization necessary. The kit is flexible enough to allow for scaling down reaction volumes to half the recommended quantities, without impacting library quality. Easier to use than Illumina DNA prep and equivalent to SPARQ DNA library prep.
Review Date: 26 Nov 2024 | Twist Bioscience
Population genetics
The EF 2.0 library prep kit is very easy to use. The instructions are clear and easy to follow, and the troubleshooting guides in the appendix are helpful. Instructions with optional stopping points and tips for success make it easy to plan your lab time. I have applied this kit to a few hundred samples now and plan to continue. Everything work as expected and I am using it for non-model organisms and difficult specimen types (dung).
Review Date: 2 Feb 2023 | Twist Bioscience
Analyze germline genetic testing
Very happy with the quality of results we generate with this product. One of the best in the market!
Review Date: 9 Jan 2022 | Twist Bioscience
Twist Library Preparation Enzymatic Fragmentation (EF) Kit 2.0 is built to help you achieve a more efficient sequencing pipeline and obtain more accurate Next-Generation Sequencing (NGS) results. This updated kit has new fragmentation and ligation modules, and includes the Equinox Library Prep Amp Mix. This hot-start enzyme formulation has a lower error rate* and high efficiency at low input volumes—making it ideal for samples such as FFPE, where high efficiency and yield are necessary.