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Twist Library Preparation EF Kit (Enzymatic Fragmentation)

Twist Library Preparation EF Kit (Enzymatic Fragmentation) incorporates the reagents and enzymes needed for DNA fragmentation with those for end repair, dA-tailing, and adapter ligation into a single reaction:  Ideal for automated, high-throughput library preparation, produces tunable, reproducible fragment sizes and minimizes sequence bias, maximizes coverage depth.

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Twist Bioscience

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Spec:

  • 16 Samples
  • 96 Samples

KEY BENEFITS:

Accommodate Either Enzymatic or Mechanical Fragmentation

  • Use enzymatic fragmentation for automated, high-throughput gDNA library construction
  • Use mechanical fragmentation for DNA input of varying types and quality

Accommodate Either Whole Genome Sequencing or Targeted Enrichment

  • Use with either Twist full-length CD adapters for PCR-free library preparation
  • Use with the Twist Universal Adapter (UDI) System for maximum yield

Streamline Your Workflow

  • Optimized for use in the Twist Targeted NGS workflow
  • Flexible DNA sample input from 1 ng – 1 µg (enzymatic)
  • Convert DNA samples into robust, amplified libraries in under 2.5 hours
  • Combine library preparation steps into a single reaction for improved efficiency and consistent results

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