Twist RNA Library Prep with Depletion
Twist's RNA Sequencing Solutions provide comprehensive next-generation sequencing (NGS) workflows, offering both targeted and whole transcriptome approaches to generate uniform, high-quality RNA sequencing libraries from diverse sample types.
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Twist Bioscience offers a suite of RNA sequencing solutions designed to streamline NGS workflows and produce consistent, high-quality libraries suitable for various research applications. These solutions cater to both targeted RNA sequencing and whole transcriptome sequencing, accommodating a wide range of inputs and sample types, including challenging specimens like formalin-fixed paraffin-embedded (FFPE) tissues.
By combining the Twist RNA Library Prep Kit with Twist Hybridization and RNA target enrichment panels, users can enhance sensitivity for key targets, even in low-quality or degraded RNA samples. Twist also provides custom panels that allow researchers to focus on specific RNA transcripts of interest.
In whole transcriptome sequencing applications, Twist's workflow enables the measurement of expression levels of various RNA species, including mRNA and long non-coding RNA (lncRNA). The process involves preparing libraries from total RNA extracted from fresh or FFPE samples, utilizing the Twist RNA Library Prep Kit in conjunction with the Twist rRNA & Globin Depletion Kit. This combination effectively removes abundant ribosomal and globin RNA, resulting in more informative sequencing data.
Additionally, Twist offers solutions for RNA fusion detection, such as the Twist Alliance CeGaT Fusion Panel. This panel enriches for both known and novel gene fusions, leveraging expert content design through collaboration with CeGaT and optimized workflows with the Twist RNA Library Prep Kit to produce sequencing-ready libraries.
Key features:
- Comprehensive Workflows: End-to-end solutions encompassing library preparation, target enrichment, and optimized panel designs for both targeted and whole transcriptome sequencing.
- Customizable Panels: Ability to design custom RNA panels targeting specific transcripts, enhancing sensitivity and focus in sequencing projects.
- Efficient Depletion: Effective removal of unwanted ribosomal and globin RNA using the Twist rRNA & Globin Depletion Kit, improving data quality.
- Expert Collaboration: Access to specialized panels like the Twist Alliance CeGaT Fusion Panel for comprehensive fusion detection.
Applications:
- Gene Expression Profiling: Quantification of RNA transcripts to study gene expression patterns across different conditions or treatments.
- Transcriptome Analysis: Comprehensive examination of all RNA species within a sample, facilitating the discovery of novel transcripts and splice variants.
- Fusion Gene Detection: Identification and characterization of gene fusions, which are critical in various cancers and genetic disorders.
- Targeted RNA Sequencing: Focused analysis of specific RNA transcripts of interest, allowing for in-depth study of particular genes or pathways.
For research use only. Not for use in diagnostic procedures.
