Twist UMI Adapter System

The Twist UMI Adapter System is designed to improve the accuracy of next-generation sequencing (NGS) in identifying rare somatic mutations, particularly in cfDNA samples. By integrating UMIs—short, unique sequences added to each DNA fragment—this system enables the differentiation between true low-frequency variants and errors introduced during PCR amplification. This approach allows for the construction of consensus reads, thereby increasing variant detection sensitivity and minimizing false positives. The system includes matched UMI adapters compatible with 'T-A' overhang workflows and 10 bp unique dual-indexed (UDI) primers, provided in a 96-well plate format suitable for automation. Available in configurations for 16 or 96 samples, the Twist UMI Adapter System is compatible with multiple Twist library preparation kits and has been tested for performance with Twist's target enrichment panels.

Key features:

• High Sensitivity: Matched adapters enable superior identification of PCR errors, reducing false positives of low-frequency variants for cancer research and discovery.
• Compatibility: Easily integrates with Twist’s NGS solutions, including library preparation workflows and target enrichment panels.
• Scalability: Suitable for various somatic applications, accepting gDNA or cfDNA input, and available in scalable formats for 16 or 96 samples.

Applications:

• Cancer Research: Enhances the detection of rare somatic mutations in cfDNA, aiding in the study of tumor genetics and heterogeneity.
• Liquid Biopsy Research: Improves the sensitivity and accuracy of assays designed to detect circulating tumor DNA (ctDNA) in plasma samples.
• Genomic Studies: Facilitates the identification of low-frequency variants in heterogeneous samples, supporting various genomic research initiatives.

For research use only. Not for use in diagnostic procedures.